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KMID : 0191119980130050507
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Journal of Korean Medical Science
1998 Volume.13 No. 5 p.507 ~ p.512
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p21 expression and mutation in gastric carcinoma: analysis by immunohistochemistry and PCR-SSCP
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Park YE
Choi KC/Choi YH
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Abstract
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p21 protein has been reported to be a critical downstream effector of p53 and a potent inhibitor of
cyclin-dependent kinases. Thus, the p21 gene is thought to play a central role in tumor suppression. In
this study we investigated p21 protein expression and mutation in gastric adenocarcinoma. A total of 76
primary gastric carcinoma specimens were immunohistochemically stained for p21 protein expression and
evaluated the correlations between p21 expression and clinicopathologic features. In a proportion of
them (20 cases), we also analyzed the possible presence of p21 gene mutations using PCR-SSCP method.
Fourty seven out of 76 cases (61.8%) were p21-negative, and the remaining twenty nine cases (38.2%)
were p21 -positive on immunostains. There was a correlation between the expression of p21, and the depth
of tumor invasion and lymph node metastasis (p<0.05). No mutation of the p21 gene was detected in all of
20 tumor tissues. These results suggest that the status of p21 expression may have prognostic value in
gastric adenocarcinoma.
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KEYWORD
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